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Image Search Results
Journal: The Plant journal : for cell and molecular biology
Article Title: Subdiffraction-resolution live-cell imaging for visualizing thylakoid membranes
doi: 10.1111/tpj.14021
Figure Lengend Snippet: Chl fluorescence from isolated A. thaliana chloroplasts observed by 3D-SIM. Optical sections of chloroplasts isolated from (a) WT, (b) stn7 stn8, and (c) tap38 are shown whole (left column) and enlarged to show detail (right column). Each number corresponds to the selected focal plane of the serial optical sections as shown in Figures S1–3. Scale bars, 1 µm. (d) Representative close-up image of a chloroplast side-view with a line scan for a thickness analysis of thylakoid membranes. (e) The intensity profile of the line indicated in (d) (top panel). The dotted line is the multipeak fit, which is used to extract the Gaussian distribution of each peak (bottom panel). The number indicates FWHM (µm) measured in each Gaussian distribution. (f) Thylakoid membrane length in chloroplasts of WT (n = 173 from 8 chloroplasts), stn7 stn8 (n = 144 from 8 chloroplasts), and tap38 (n = 156 from 8 chloroplasts). Inset numbers indicate mean FWHM ± SD of all chloroplasts, and data represent means ± SD for each chloroplast.
Article Snippet: Peak fitting and FWHM measurements were done using the
Techniques: Fluorescence, Isolation
Journal: The Plant journal : for cell and molecular biology
Article Title: Subdiffraction-resolution live-cell imaging for visualizing thylakoid membranes
doi: 10.1111/tpj.14021
Figure Lengend Snippet: Chl fluorescence from C. reinhardtii chloroplasts observed by 3D-SIM. As compared to reconstructed widefield images (a,c), reconstructed 3D-SIM images (b,d) of C. reinhardtii chloroplasts revealed the distinct thylakoid membrane structures. Arrows and arrowheads in (d) indicate the basal and lobe regions, respectively. (e) Selected optical sections of close-up 3D-SIM images showing the basal region of the chloroplast as shown in (d). Each number corresponds to the selected focal plane of the serial optical sections as shown in Figure S4. Arrows in (e) indicate thylakoid membranes, which appeared to extend into the pyrenoid. (f) Detail image of the thylakoid tip convergence region. (g) The intensity profile of the line indicated in (f). The dotted line is the multipeak fit, which was used to extract the Gaussian distribution of each peak as shown at the bottom. The number indicates FWHM (nm) measured in each Gaussian distribution. Scale bars, 10 µm (a,b), 2 µm (c–f).
Article Snippet: Peak fitting and FWHM measurements were done using the
Techniques: Fluorescence